Press Release Archive

  • home_icon HOME
  • News
  • Press Release Archive

KAIST researchers have developed a revolutionary bio-medical imaging tool, the HT-1, to view and analyze cells, which is commercially available.

 

Professor YongKeun Park of the Physics Department at the Korea Advanced Institute of Science and Technology (KAIST) and his research team have developed a powerful method for 3D imaging of live cells without staining. The researchers announced the launch of their new microscopic tool, the holotomography (HT)-1, to the global marketplace through a Korean start-up that Professor Park co-founded, TomoCube (www.tomocube.com).


Professor Park is a leading researcher in the field of biophotonics and has dedicated much of his research career to working on digital holographic microscopy technology. He collaborated with TomoCube’s R&D team to develop a state-of-the-art, 2D/3D/4D holographic microscope that would allow a real-time label-free visualization of biological cells and tissues.

 

The HT is an optical analogy of X-ray computed tomography (CT). Both X-ray CT and HT share the same physical principle—the inverse of wave scattering. The difference is that HT uses laser illumination whereas X-ray CT uses X-ray beams. From the measurement of multiple 2D holograms of a cell, coupled with various angles of laser illuminations, the 3D refractive index (RI) distribution of the cell can be reconstructed. The reconstructed 3D RI map provides structural and chemical information of the cell including mass, morphology, protein concentration, and dynamics of the cellular membrane.

 

The HT enables users to quantitatively and non-invasively investigate the intrinsic properties of biological cells, for example, dry mass and protein concentration. Some of the research team’s breakthroughs that have leveraged HT’s unique and special capabilities can be found in several recent publications, including a lead article on the simultaneous 3D visualization and position tracking of optically trapped particles which was published in Optica on April 20, 2015.

 

Current fluorescence confocal microscopy techniques require the use of exogenous labeling agents to render high-contrast molecular information. Therefore, drawbacks include possible photo-bleaching, photo-toxicity, and interference with normal molecular activities. Immune or stem cells that need to be reinjected into the body are considered particularly difficult to employ with fluorescence microscopy.

 

“As one of the two currently available, high-resolution tomographic microscopes in the world, I believe that the HT-1 is the best in class regarding specifications and functionality. Users can see 3D/4D live images of cells, without fixing, coating or staining cells. Sample preparation times are reduced from a few days or hours to just a few minutes,” said Professor Park.

 

Two Korean hospitals, Seoul National University Hospital in Bundang and Boramae Hospital in Seoul, are using this microscope currently. The research team has also introduced the HT-1 at the Photonics West Exhibition 2016 that took place on February 16-18 in San Francisco, USA.

 

Professor Park added, “Our technology has set a new paradigm for cell observation under a microscope. I expect that this tomographic microscopy will be more widely used in future in various areas of pharmaceuticals, neuroscience, immunology, hematology, and cell biology.”



Figure 1: HT-1 and Its Specifications

H_20160329171317_420.jpg


Figure 2: 3D Images of Representative Biological Cells Taken with the HT-1

3_20160329171347_559.jpg


List of Articles
No. Category Subject Author Date Views
» KIHST Next-generation Holographic Microscope for 3D Live Cell Imaging [2016-03-29] file KAIST_INSTITUTE 2016.06.18 17039
34 KINC KAIST Develope a Credit-Card-Thick Flexible Lithium Ion Battery [2015-08-06] file KAIST_INSTITUTE 2016.06.10 14477
33 KIHST Fast and Accurate 3-D Imaging Technique to Track Optically-Trapped Particles [2015-08-06] file KAIST_INSTITUTE 2016.06.10 12971
32 Others A Firefighter Drone That Flies and Crawls Up Walls [2016-01-20] file KAIST_INSTITUTE 2016.06.18 11764
31 KIHST Anti-Cancer Therapy Delivering Drug to an Tumor Developed by KAIST Researchers [2015-08-06] file KAIST_INSTITUTE 2016.06.10 8821
30 KIB Prof. Sang-Yup Lee's Team Pioneers Biotechnological Production of Chemical Using Renewable Materials [2009-11-02] KAIST_INSTITUTE 2016.06.09 8185
29 KIB Appointed as Head Director of the Global Frontier Program (GFP) of National Research Foundation [2012-01-12] file KAIST_INSTITUTE 2016.06.10 8119
28 KIB Professor Sang-Yup Lee Named Senior Editor of U.S. Biotechnology Journal [2007-02-07] KAIST_INSTITUTE 2016.06.08 7804
27 KINC Graphene-based Transparent Electrodes for Highly Efficient Flexible OLEDs [2016-06-07] file KAIST_INSTITUTE 2016.06.18 7624
26 Others KIUSS Research Contract on the Branch of 'Smart Green City' [2012-01-12] file KAIST_INSTITUTE 2016.06.10 7522
25 Others 융합연구기획과제 성과 - 대학 캠퍼스 내 실내외 통합 내비게이션 개발 [2015-09-17] file KAIST_INSTITUTE 2016.06.10 7500
24 KINC KAIST Researchers Develop a Technology to Enable Unzipping of the Graphene Plane [2016-03-22] file KAIST_INSTITUTE 2016.06.18 7089
23 KINC 최성율 교수 도장 찍듯이 자유롭게 그래핀 옮기는 기술 개발 [2015-08-05] file KAIST_INSTITUTE 2016.06.10 6842
22 KIR BBC News Channel's Technology Program "Click"Aired KAIST's Jellyfish Robot [2015-08-06] file KAIST_INSTITUTE 2016.06.10 6813
21 KINC 조병진, 임성갑, 유승협 교수 휘어지는 10나노미터 고분자 절연막 개발 [2015-08-05] file KAIST_INSTITUTE 2016.06.10 6409
20 KIR Developes Industry's First Cargo Hold for LNG Tankers [2012-01-12] file KAIST_INSTITUTE 2016.06.10 6247
19 KIHST Ultrashort EUV Pulses generation using high harmonic generation by plasmonic field enhancement [2012-01-12] file KAIST_INSTITUTE 2016.06.10 5842
18 KIITC KAIST's Smart E-book System More Convenient than Paper-based Books [2012-02-19] file KAIST_INSTITUTE 2016.06.10 5564
17 KINC 전기적 자기적 성질 동시에 갖는 신물질 물성 규명 [2012-01-17] file KAIST_INSTITUTE 2016.06.10 5554
16 KINC 보다 태양빛에 가까운 LED 개발 [2012-01-12] file KAIST_INSTITUTE 2016.06.10 5225
Board Pagination Prev 1 2 Next
/ 2

KAIST 291 Daehak-ro, Yuseong-gu, Daejeon (34141)
T : +82-42-350-2381~2384
F : +82-42-350-2080
Copyright (C) 2015. KAIST Institute